Description
Synonyms: MH Broth; Antibacterial potency testing media; Antimicrobial susceptibility testing media
General description
Mueller Hinton Broth is used for determining minimal inhibitory concentrations (MICs). Mueller Hinton medium is recommended by FDA, World Health Organization, and NCCLS for testing the most encountered aerobic and facultative anaerobic bacteria in food and clinical material. The medium shows good batch-to-batch reproducibility, it is low in sulfonamide, trimethoprim, and tetracycline inhibitors and yields satisfactory growth of most non-fastidious pathogens.
Application
Mueller Hinton broth is primarily used to determine the MIC (minimum inhibitory concentration) for several antimicrobial agents. It can also be used to cultivate fastidious organisms like Meningococcus and Gonococcus
PROPERTIES
sterility
non-sterile
Pack Size
500g
product line
BioChemika
form
powder
shelf life
limited shelf life, expiry date on the label
composition
beef infusion solids, 2.0 g/L
casein hydrolysate, 17.5 g/L
starch, 1.5 g/L
manufacturer/tradename
NutriSelect® Plus
storage condition
protect from light
technique(s)
microbe id | susceptibility testing: suitable
microbiological culture: suitable
final pH
7.4±0.2 (25 °C)
application(s)
clinical testing
food and beverages
microbiology
storage temp.
15-25°C
suitability
nonselective for bacteria (General Media)
Preparation Note
Dissolve 21 g in 1 litre of distilled water. Sterilize by autoclaving at 121°C for 15 minutes.
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