Description
Synonym: 4-Methylumbelliferyl β-D-Glucuronide Escherichia coli Broth
DESCRIPTION
Application
Used for the detection of E. coli by a fluorogenic procedure.
Components
Ingredients (g/L)
Casein enzymatic hydrolysate, 20.00
Lactose, 5.00
Bile salts mixture, 1.50
Dipotassium phosphate, 4.00
Monopotassium phosphate, 1.50
Sodium chloride, 5.00
4-Methylumbeliferyl β-D-glucuronide (MUG), 0.05
Casein enzymatic hydrolysate, 20.00
Lactose, 5.00
Bile salts mixture, 1.50
Dipotassium phosphate, 4.00
Monopotassium phosphate, 1.50
Sodium chloride, 5.00
4-Methylumbeliferyl β-D-glucuronide (MUG), 0.05
Preparation Note
Suspend 37 g of MUG EC Broth in 1000 ml of distilled water. Dispense in tubes containing inverted Durham′s tubes. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 12-15 minutes.
PROPERTIES
sterility
non-sterile
Pack Size
500g
form
powder
shelf life
limited shelf life, expiry date on the label
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
final pH
6.9±0.2 (25 °C)
application(s)
agriculture
environmental
food and beverages
veterinary
water monitoring
microbiology
suitability
selective and differential for Escherichia coli
selective and differential for coliforms
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