Description
Application
Mueller Kauffman Tetrathionate Novobiocin Broth is used for improved enrichment and isolation of Salmonellae.
Preparation Note
Suspend 89.42 grams of dehydrated mediumI in 1000 ml distilled water. Heat the medium just to boiling. DO NOT AUTOCLAVE. Cool to 45-50°C and just before use aseptically add 20 ml of iodine solution (20 gram iodine and 25 gram potassium iodide in 100 ml sterile distilled water) along with rehydrated contents of 1 vial of MKTT Novobiocin Supplement. Mix well to disperse calcium carbonate uniformly before dispensing in sterile tubes.
Note: Due to presence of calcium carbonate, the prepared media forms opalescent solution with white precipitate.
Note: Due to presence of calcium carbonate, the prepared media forms opalescent solution with white precipitate.
PROPERTIES
sterility
non-sterile
form
powder
shelf life
limited shelf life, expiry date on the label
composition
brilliant green, 0.0095 g/L
calcium carbonate, 38.7 g/L
Casein enzymic hydrolysate, 8.6 g/L
ox bile, 4.75 g/L
peptic digest of animal tissue, 4.3 g/L
sodium chloride, 2.6 g/L
sodium thiosulphate, pentahydrate, 47.8 g/mL
manufacturer/tradename
NutriSelect® Plus
final pH
8.2±0.2 (25 °C)
application(s)
environmental
food and beverages
sample preparation
microbiology
suitability
Salmonella spp.
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