Description
BioReagent, suitable for electrophoresis, ~99%
Synonyms: 1,2-Bis(dimethylamino)ethane, TEMED, TMEDA
Linear Formula: (CH3)2NCH2CH2N(CH3)2
General description
TEMED (N,N,N′,N′-Tetramethylethylenediamine) is molecule which allows rapid polymerization of polyacrylamide gels. In the presence of ammonium persulfate, TEMED is responsible for the formation of free radicals from persulfate, thereby initiating the acrylamide polymerization process. However, excess concentration of TEMED can result in abnormal separation patterns.
Application
TEMED (N,N,N′,N′-Tetramethylethylenediamine) has been used for the preparation of acrylamide hydrogels and sodium dodecyl sulfate-polyacrylamide gels.
Caution
Handle and store under inert gas, air and moisture sensitive.
PROPERTIES
product line
BioReagent
Assay
~99%
expl. lim.
9.08 %
technique(s)
electrophoresis: suitable
refractive index
n20/D 1.4179 (lit.)
bp
120-122 °C (lit.)
mp
−55 °C (lit.)
density
0.775 g/mL at 20 °C (lit.)
suitability
suitable for electrophoresis
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