This culture medium is in accordance with the recommendations of the Institut für Lebensmitteltechnologie und Verpackung (Institute for Food Technology and Packaging) (1974).
Mode of Action
The culture medium is optimally adapted to the special requirements of the microbial flora present in citrus juices (e.g.Bacillus, Lactobacillus, Leuconostoc species, fungi, etc.) due to the fact that it contains orange extract. It is therefore especially useful for the production control in the fruit juice industry (HAYS and TIESTER 1952).
Typical Composition (g/litre)
Peptone from casein 10.0; yeast extract 3.0; orange extract 5.0; D(+)glucose 4.0; di-potassium hydrogen phosphate 3.0; agaragar 17.0.
Suspend 42 g/litre, autoclave under mild conditions (15 min at115 °C). Do not overheat. Pour plates.
pH: 5.5 ± 0.2 at 25 °C.
The plates are clear to opalescent and yellowish-brown
Experimental Procedure and Evaluation
Incubate the inoculated culture medium for up to 4 days at 28 °C aerobically; if fungi are suspected to be present, incubate for up to 5 days. Determine the microbial count. Further tests can be performed to differentiate and identify the colonies