Description
Medium proposed by HIRSCH and GRINSTED (1954) for the cultivation and enumeration of clostridia, other anaerobes
and facultative microorganisms in foodstuffs, clinical specimens and other materials.
Preparation
Dissolve 33 g/litre, dispense into test tubes, autoclave (15 min at 121 °C). Cool, if required add 0.02 g Polymyxin B/litre in form of an aqueous solution and mix.
pH: 6.8 ± 0.2 at 25 °C.
The prepared medium in the tubes is clear and yellowish.
Experimental Procedure and Evaluation
After inoculation it is advised to cover the medium with a layer of paraffin viscous or agar.
Incubation: 24-48 hours at 35 °C aerobically.
Count the colonies which have grown and, if necessary, perform further tests
PROPERTIES
Agency
according to EP
according to JP
according to USP
Pack Size
500g
form
medium granules (dehydrated (DCM))
packaging
pkg of 500 g
manufacturer/tradename
GranuCult® prime
storage condition
dry media (Keep tightly closed)
technique(s)
microbiological culture: suitable
application(s)
food and beverages
microbiology
storage temp.
15-25°C
suitability
Clostridium spp.
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