VRB Agar (Violet Red Bile Agar)

$275.62

Selective agar proposed by DAVIS (1951) for the detection and enumeration of coliform bacteria including E. coli in
water, milk, ice-cream, meat and other foodstuffs.

Available on backorder

SKU: ZA.002.038 Category:

Description

This medium complies with the recommendations of the American Public Health Association (1992), the International Dairy Federation FIL-IDF (Internationaler Milchwirtschaftsverband 1985), the Institute for Food Technology and Packaging (Institut für Lebenmitteltechnologie und Verpackung) (1974) and
the EUROGLACE (KLOSE 1968 a, b).


Mode of Action
Crystal violet and bile salts inhibit growth primarily of the Grampositive accompanying bacterial flora. Degradation of lactose to acid is indicated by the pH indicator neutral red, which changes its colour to red, and by precipitation of bile acids.


Typical Composition (g/litre)
Peptone from meat 7.0; yeast extract 3.0; sodium chloride 5.0; lactose 10.0; neutral red 0.03; bile salt mixture 1.5; crystal voilet 0.002; agar-agar 13.0.


Preparation
Suspend 39.5 g in 1 litre of demin. water and heat to boiling with frequent stirring until completely dissolved. Afterwards do not boil more than 2 minutes. Do not autoclave. Do not overheat!
pH: 7.4 ± 0.2 at 25 °C.
The prepared medium is clear and dark-red.


Experimental Procedure and Evaluation
Culture medium is usually inoculated by the pour-plate procedure.
Incubation: 24 ± 2hours at 30 ± 1 °C (IDF-FIL) respectively according to recommended procedures.


Literature
American Public Health Association: Compendium of Methods for the microbiological Examination of Foods. – 3rd ed. (1992).
American Public Health Association: Standard Methods for the Examination of Dairy Products. – 15
th ed. (1995).
DAVIS, J.G.: Milk Testing –
Dairy Industries Ltd., London, 1951.
Institut für Lebensmitteltechnologie und Verpackung der TU München:
Merkblatt 19: Bestimmung der Gesamtkeimzahl, der Anzahl an Schimmelpilzen und Hefen und der Anzahl an coliformen Keimen in Flaschen und vergleichbaren enghalsigen Behältern. –
Milchwiss., 29; 602-606 (1974).
Internationaler Milchwirtschaftsverband: Zählung coliformer Bakterien in Milch und Milchprodukten. –
Internationaler Standard FIL-IDF 73 A: 1985.
KLOSE, J.: Harmonisierung des Speiseeisrechtes in der EWG. –
Süßwaren,14; 778-780 (1968a).
KLOSE, J.: Entwurf einer Richtlinie zur Angleichung der Rechtsvorschriften
für Speiseeis in den Mitgliedsstaaten der EWG. Neufassung des AnhangsIII
zum Entwurf vom 19.12.1966. –
Süßwaren, 14; 780-782 (1968b).
Escherichia coli
ATCC 11775

Appearance of Colonies Microorganisms
Red, surrounded by reddish
precipitation zones, diameter
1-2 mm
Lactose-positive Enterobacte
riaceae: coliform bacteria, E.
coli
Pink pin-point colonies Enterococci, possibly Klebsiella
Colourless Lactose-negative Enterobacte
riaceae

 

 

Additional information

Weight 0.5 kg

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