Reticulocyte Stain

Utilizes New Methylene Blue for the identification of reticulocytes in blood films. Reticulocyte Stain has been used in the detection of reticulocytes.

Blood and reticulocyte staining solution (New Methylene Blue) are mixed and incubated briefly at room temperature. Wedge or spun smears are made on microscope slides, air dried and evaluated under oil immersion on a light microscope. A red blood cell scoring positive would be observed containing two or more blue-stained granules.