Description
For the isolation of salmonellae and shigellae from faeces, foodstuffs and other materials.
Principle
Microbiological method
Mode of Action
Brilliant green, ox bile and high concentrations of thiosulfate and citrate largely inhibit the accompanying microbial flora.
Sulfide production is detected by using thiosulfate and iron ions, the colonies turn black. The presence of coliform bacteria is established by detecting degradation of lactose to acid with the pH indicator neutral red.
Typical Composition (g/litre)
Peptones 10.0;
lactose 10.0;
ox bile 8.5;
sodium citrate 10.0;
sodium thiosulfate 8.5;
ammonium iron(III) citrate 1.0;
brilliant green 0.0003;
neutral red 0.025;
agar-agar 12.0.
Preparation and Storage
Usable up to the expiry date when stored dry and tightly closed
at +15 to +25° C. Protect from light.
After first opening of the bottle the content can be used up to the
expiry date when stored dry and tightly closed at +15 to +25°C.
Suspend 60 g/litre completely, pour plates.
n Do not autoclave.
pH: 7.0 ± 0.2 at 25 °C.
The plates are clear and reddish-brown.
Reviews
There are no reviews yet.