Description
General description
SDS-PAGE Clean-Up Kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyze due to the presence of salts or Low Protein concentration.
Application
The SDS-PAGE Clean-Up Kit uses a combination of a precipitant and co-precipitant to quantitatively precipitate the sample Proteins while leaving interfering substances in solution. Detergents, salts, lipids, phenolics, and nucleic acids remain soluble while the Proteins are pelleted by centrifugation. The precipitate is washed further to remove nonProtein contaminants and centrifuged again. The resultant pellet is resuspended, mixed with SDS-PAGE sample buffer, and heated. The sample is then ready for SDS-PAGE. The procedure can be completed in under 2 h with quantitative yield.
Features and Benefits
- Prepares samples for SDS-PAGE that are otherwise difficult to analyze because of salts or Low Protein concentrations
- Quantitatively precipitate Proteins while leaving interfering substances in solution
- Delivers quantitative yield in less than 2 h.
PROPERTIES
usage
sufficient for 50 preparations
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
feature
wetted part: no
manufacturer/tradename
Cytiva 80-6484-70
packaging
pkg of 50 samples
storage condition
room temperature
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