Description
General description
Thiosulfate citrate bile salts sucrose (TCBS) Agar uses an alkaline pH (8.6), ox bile and 1% NaCl to prevent the growth of gram positive bacteria, members of the Enterobacteriaceae family and the genus Pseudomonas.
Application
Thiosulfate citrate bile salts sucrose (TCBS) Agar is a selective agar for the isolation and cultivation of Vibrio cholerae and other Vibrio species.
Preparation Note
Dissolve 88 g in 1 litre distilled water and bring to the boil. Pour plates. Do NOT autoclave.
Other Notes
Incidence of Vibrio cholerae from estuaries of the United States West Coast; Survival and multiplication of Vibrio cholerae serotype Ogawa in reconstituted infant milk.
PROPERTIES
Agency
according to ISO 21872-1:2017
according to ISO 21872-2:2007
Pack Size
500 g
sterility
non-sterile
form
powder
shelf life
limited shelf life, expiry date on the label
composition
agar, 14 g/L
bromothymol blue, 0.04 g/L
ferric citrate, 1 g/L
mixed peptone, 10 g/L
ox-bile, 5 g/L
sodium chloride, 10 g/L
sodium cholate, 3 g/L
sodium citrate, 10 g/L
sodium thiosulfate, 10 g/L
sucrose, 20 g/L
thymol blue, 0.04 g/L
yeast extract, 5 g/L
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
final pH
8.6±0.2 (25 °C)
application(s)
environmental
food and beverages
microbiology
suitability
selective and differential for Vibrio spp.
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