Description
DESCRIPTION
TSI Agar Culture medium proposed by SULKIN and WILLETT (1940) and modified by HAJNA (1945) for identifying Enterobacteriaceae.
This medium complies with the recommendations of the International Organization for Standardization (ISO) (1975), DIN Norm 10160 for the examination of meat and DIN Norm 10181 for the examination of milk. Its composition is equivalent to that recommended by the United States Pharmacopeia XXVI (2003), the European Pharmacopeia II and the German examination procedure for food acc. to § 35 LMBG.
Mode of Action
Degradation of sugar and accompanying acid production are detected by the pH indicator phenol red, which changes its colour from red-orange to yellow, on alkalinization it turns deep red. Thiosulfate is reduced to hydrogen sulfide by several species of bacteria, the hydrogen sulfide reacts with an iron salt to give black iron sulfide.
Application
Preparation Note
PROPERTIES
Agency
according to ISO 19250:2010
according to ISO 21567:2004
according to ISO 6579-1:2020
according to ISO 6579-2:2012
according to ISO 6579-3:2014
Pack Size
500g
form
powder
shelf life
limited shelf life, expiry date on the label
composition
agar, 12.000 g/L
glucose, 1.000 g/L
iron(III) citrate, 0.300 g/L
lactose, 10.000 g/L
meat extract, 3.000 g/L
peptone, 20.000 g/L
phenol red, 0.024 g/L
sodium chloride, 5.000 g/L
sodium thiosulfate, 0.300 g/L
sucrose, 10.000 g/L
yeast extract, 3.000 g/L
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbe id | utilization test: suitable
final pH
7.4±0.2 (25 °C)
application(s)
environmental
food and beverages
pathogen testing
microbiology
suitability
Citrobacter spp.
Enterobacter spp.
Escherichia coli
Klebsiella spp.
Proteus spp.
Salmonella spp.
Shigella spp.
coliforms
enterobacteriaceae
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