Description
Synonyms: CASO Agar, Soybean Casein digest Agar, TSA,Tryptone Soya Agar
General description
Tryptic Soy Agar is a general-purpose culture medium for the isolation and cultivation of fastidious and non-fastidious microorganisms or the maintenance of stock culture. It is a complex medium for the cultivation and isolation of a wide range of bacteria, yeasts, and molds. The formulation of the TSA medium contains a combination of casein and soy peptones supplying organic nitrogen, particularly amino acids, and longer-chained peptides. Sodium chloride maintains the osmotic equilibrium, and the natural sugars from soy peptone are the energy source.
Application
Tryptic Soy Agar is used for the pre-cultivation and enumeration (E. coli) by membrane filtration technique. It is suitable for the cultivation of both aerobic and anaerobic bacteria. Since it does not contain the X and V factors, it is suitable for the identification of Haemophilus sp. by adding X (Hemin) and V (DPN=NAD) factors strips.
Preparation Note
Suspend 40 g of dehydrated media in 1 litre of purified filtered water. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.
PROPERTIES
Agency
according to GB 4789.40-2016
according to ISO 11731:2017
according to ISO 22964:2017
sterility
non-sterile
product line
BioChemika
form
powder
shelf life
limited shelf life, expiry date on the label
composition
agar, 15 g/L
casein peptone (pancreatic), 15 g/L
sodium chloride, 5 g/L
soya peptone (papainic), 5 g/L
packaging
bottle of 2.5 kg
bottle of 500 g
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbe id | specific enzyme detection: suitable
microbiological culture: suitable
color
light yellow
final pH
7.3±0.2 (25 °C)
application(s)
agriculture
bioburden testing
cleaning products
clinical testing
cosmetics
environmental
food and beverages
personal care
surface monitoring
veterinary
microbiology
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
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