Tryptone water 500g

Description

For the detection of microbial indole formation when identifying microorganisms by biochemical methods.

This culture medium is recommended by the International Organization for Standardization (ISO) (1975) for the detection of E. coli in the examination of meat and meat products. It can be used instead of Tryptophane Broth recommended in the Standard Methods for the Examination of Water and Wastewater (1992). The medium also complies with the APHA (1998) recommendations for food examination.

Mode of Action
Peptone from casein (= tryptone) contains a high proportion of tryptophane which is degraded by indole-positive organisms to form indole. Indole can be detected with KOVACS Indole Reagent.

Typical Composition (g/litre)
Peptone from casein 10.0; sodium chloride 5.0.

Preparation
Suspend 15 g/litre, dispense into tubes, autoclave (15 min at 121°C).
pH: 7.3 ± 0.2 at 25 °C.
The prepared broth is clear and yellowish.