Anaerobic agar, Wilkins Chalgren 500g

Description

Application
Used for the isolation of anaerobic bacteria.

Components
Ingredients (g/L)
Casein enzymatic hydrolysate, 10.00
Peptic digest of animal tissue, 10.00
Yeast extract, 5.00
Dextrose, 1.00
Sodium chloride, 5.00
L-Arginine, 1.00
Sodium pyruvate, 1.00
Hemin, 0.005
Menadione, 0.0005
Agar, 10.00

Preparation Note
Suspend 43 g of Wilkins Chalgren Anaerobic Agar in 1000 ml of distilled water. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 50°C before adding antibiotics to be tested. Mix gently and pour into sterile petri plates .For the cultivation of anaerobes add 2 vial of Non-Spore Anaerobic Supplement or 2 vial of G.N. Spore Anaerobic Supplement as desired into the sterile molten medium before pouring into sterile petri plates.