Corn Meal Agar (CMA) 500g

Description

General description

Corn Meal Agar is a general-purpose medium suitable for the cultivation of fungi, studying mycelium or pseudo mycelium formation, and the study of Candida species for chlamydospore production. Corn meal infusion from maize is rich in carbon and amino acid sources, along with many other trace elements supporting the growth of fungi. The medium can be modified by adding polysorbate 80 to stimulate the formation of chlamydospores in Candida albicans, Candida stellatoides, and Candida tropicalis. Polysorbate 80 is a mixture of oleic esters, which activates the production of chlamydospores by Candida species. Some Candida species lose their ability to form chlamydospores by repeated subculturing. The addition of dextrose promotes a luxuriant growth of some fungi. Dextrose (2 g/L) in the medium is suitable for the cultivation of commonly occurring phyto pathological fungi. It also enhances the chromogenesis of some species of Trichophyton such as Trichophyton rubrum. Corn Meal Agar with dextrose should not be used for chlamydospores production.

Application
Corn Meal Agar is recommended for chlamydospore production by Candida albicans and the maintenance of fungal stock cultures.

Preparation Note
Suspend 17g in 1 litre of distilled water. Bring to the boil to dissolve completely. If desired add 1% polysorbate 80. Sterilise by autoclaving at 121°C for 15 minutes.

PROPERTIES

sterility
non-sterile

form
powder

shelf life
limited shelf life, expiry date on the label

composition
Agar, 15 g/L
Corn meal infusion (from 50 g whole maize), 2 g/L

manufacturer/tradename
NutriSelect® Plus

packaging
pkg of -500 g

storage condition
(Dry place, tightly sealed containers)

technique(s)
microbiological culture: suitable

color
light yellow

pH
6.0±0.2 (25 °C)

application(s)
bioburden testing
clinical testing
environmental
food and beverages
life science and biopharma
microbiology

storage temp.
2-25°C

suitability
nonselective for Aspergillus spp.
nonselective for Candida spp.
nonselective for Penicillium spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.
nonselective for molds (General Media)
nonselective for yeasts (General Media)