Description
Synonyms: MLGA, m-Lauryl Sulfate Chromogen Agar
Application
A chromogenic medium for the differentiation and enumeration of Escherichia coli and other coliforms, which simplifies the membrane filtration technique by reducing the number of filtration stages and the need for further confirmation steps. This medium is described in the Environment Agency′s report of UK, ′Methods for Examination of Waters and Associated Material – The Microbiology of Drinking Water (2002) – Part 4 – Methods for the isolation and enumeration of coliform bacteria and Escherichia coli (including E. coli O157:H7).
Preparation Note
Suspend 88g in 1 litre of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C and pour into sterile Petri dishes.
PROPERTIES
sterility
non-sterile
form
powder
shelf life
limited shelf life, expiry date on the label
composition
agar, 10 g/L
X-glucuronide (BCIG), 0.2 g/L
lactose, 30 g/L
peptone, 40 g/L
phenol red, 0.2 g/L
sodium lauryl sulfate, 1.0 g/L
sodium pyruvate, 0.5 g/L
yeast extract, 6 g/L
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
final pH
7.4±0.2 (25 °C)
application(s)
agriculture
bioburden testing
clinical testing
environmental
food and beverages
water monitoring
microbiology
suitability
selective and differential for Enterobacter spp.
selective and differential for Escherichia coli
selective and differential for Klebsiella spp.
selective and differential for coliforms
selective and differential for enterovirulent E. coli (EEC)
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