Description
Principle
Microbiological method
Mode of Action
The composition of the culture media provide favourable growth conditions, the media are almost totally devoid of sulfonamide
antagonists.
In order to improve the growth of fastidious microorganisms, blood can be added to MUELLER-HINTON agar. According to JENKINS et al. (1985), this may lead to false results when testing the susceptibility of enterococci to aminoglycosides.
Typical Composition (g/litre)
Meat infusion 2.0; casein hydrolysate 17.5; starch 1.5; agar-agar 13.0.
Preparation and Storage
Usable up to the expiry date when stored dry and tightly closed at +15 to +25° C. Protect from light.
After first opening of the bottle the content can be used up to the expiry date when stored dry and tightly closed at +15 to +25° C.
Suspend 34.0 g/litre, autoclave under mild conditions (10 min at 115 °C), if required cool to 45-50 °C and add 5-10 % definibrated blood, pour plates.
pH: 7.4 ± 0.2 at 25 °C.
Without blood, the plates are clear to opalescent and yellowish brown.
Specimen
e.g. Isolated bacteria from urine,
Clinical specimen collection, handling and processing, see general instructions of use.
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