Description
General description
Nutrient Broth serves as a general-purpose medium designed for the cultivation of microorganisms with minimal nutritional demands. Its nutrient composition, derived from meat infusion and peptone, forms the basis of many culture media. Nutrient Broth No. 3 is a fundamental medium utilized for maintaining microbial cultures and verifying purity. It is particularly effective for cultivating and enumerating non-fastidious bacteria. In its semisolid form, it is ideal for preserving or controlling standard microbial strains. By incorporating biological fluids such as horse or sheep blood, serum, or egg yolk, the medium can be adapted to support the growth of fastidious microorganisms.
Application
Nutrient Broth No. 3 is a liquid medium which serves as base for the culture and accumulation of microorganisms.
Preparation Note
Add 13 g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.
Properties
sterility
non-sterile
form
powder
shelf life
limited shelf life, expiry date on the label
composition
meat extract, 1 g/L , peptone, 5 g/L , sodium chloride, 5 g/L , yeast extract, 2 g/L
packaging
pack of 500 g
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
color
beige
final pH
7.4±0.2 (25 °C)
application(s)
agriculture
clinical testing
environmental
food and beverages
life science and biopharma
water monitoring, microbiology
suitability
bacteria (general purpose media), nonselective for Escherichia coli, nonselective for Pseudomonas spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for coliforms
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