Description
General description
Perfringens Agar Base with the addition of selective supplement and enrichment is used for the presumptive identification and enumeration of Clostridium perfringens from food.
Application
Perfringens Agar Base has been used in the cell culture of Clostridium perfringens.
Preparation Note
Suspend 23.5 g of Perfringens Agar Base in 475 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C. Add 25 ml of Egg Yolk Emulsion and additionally the rehydrated contents of 1 vial of Perfringens S.F.P. Supplement or 1 vial of Perfringens T.S.C. Supplement. Mix well and pour into sterile petri plates.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
PROPERTIES
sterility
non-sterile
form
powder
composition
Agar, 15 g/L
Beef extract, 5 g/L
Ferric ammonium citrate, 1 g/L
Papaic digest of soybean meal, 5 g/L
Sodium metabisulphite, 1 g/L
Tryptose, 15 g/L
Yeast extract, 5 g/L
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
application(s)
bioburden testing
cleaning products
cosmetics
environmental
food and beverages
personal care
microbiology
suitability
selective and differential for Clostridium spp.
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