The composition of this medium complies with the Standard Methods for the Examination of Water and Wastewater (1998)vand the Standard Methods for the Examination of Dairy Products (1985).
Typical Composition (g/litre)
Peptone from casein 5.0; yeast extract 2.5; D(+)glucose 1.0; agar-agar 14.0.
Suspend 22.5 g/litre, autoclave (15 min at 121 °C). If desired, add 1.0 g skim milk powder/litre prior to sterilization.
pH: 7.0 ± 0.2 at 25 °C.
The plates are clear and yellowish.
Experimental Procedure and Evaluation
Depend on the purpose for which the medium is used.
Incubation: 48 h at 30 °C aerobically.