Description
Standard Methods Agar
This medium does not contain any inhibitors or indicators; it is mainly used to determine the total microbial content in milk, dairy products, water and other materials
The composition of this medium complies with the Standard Methods for the Examination of Water and Wastewater (1998)vand the Standard Methods for the Examination of Dairy Products (1985).
Typical Composition (g/litre)
Peptone from casein 5.0; yeast extract 2.5; D(+)glucose 1.0; agar-agar 14.0.
Preparation
Suspend 22.5 g/litre, autoclave (15 min at 121 °C). If desired, add 1.0 g skim milk powder/litre prior to sterilization.
pH: 7.0 ± 0.2 at 25 °C.
The plates are clear and yellowish.
Experimental Procedure and Evaluation
Depend on the purpose for which the medium is used.
Incubation: 48 h at 30 °C aerobically.
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