Description
Synonyms: D(+)-Saccharose, α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, Sugar, β-D-Fructofuranosyl-α-D-glucopyranoside
Empirical Formula (Hill Notation): C12H22O11
General description
Sucrose is used to prepare density gradients for cell/organelle separation. In addition, sucrose can be used as a supplement in plant, insect, and bacterial culture media. It can also be used in various enzymatic assays.
Sucrose, a disaccharide composed of glucose and fructose, serves as an energy source in various biological processes. In cell biology, sucrose acts as a cryoprotectant, preserving cell viability during freezing and thawing. It is also added as a supplement in culture media, aiding in maintaining osmotic balance and enhancing cell survival.In molecular biology, sucrose is crucial in gradient solutions for DNA and RNA purification and in density centrifugation for organelle separation.
Application
Sucrose has been used:
- to perform cryoprotection for kidney tissues
- as a component in the solid germination medium to germinate pollen grains
- in the triphenyl-tetrazolium chloride (TTC) test to check the viability of pollen grains
- in the cryoprotection of fixed brain tissues for immunohistochemistry
Biochem/physiol Actions
Sucrose is used as a transport and storage molecule in most plants due to its limited chemical reactivity. It can be used to prepare density gradients for the separation of cell/organelle. Sucrose can be used in the plant, insect, and bacterial culture media as a supplement. In addition, sucrose can also be used in several enzymatic assays.
Features and Benefits
- Ideal for Molecular Biology, Cell Biology and Biochemical research
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Tested for DNase, RNase, and proteases
- Molecular biology grade sucrose, sourced from sugar cane
PROPERTIES
biological source
sugar cane
Pack Size
500g
grade
for molecular biology
Assay
≥99.5% (GC)
form
crystals
technique(s)
gas chromatography (GC): suitable
impurities
≤0.1% free glucose
color
white
useful pH range
5.5-7 (25 °C, 342 g/L)
mp
185-187 °C (lit.)
solubility
H2O: soluble 50 g + 50 mL
cation traces
heavy metals (as Pb): ≤5 ppm
absorption
≤0.15 at 280 at 50%
≤0.20 at 260 at 50%
application(s)
agriculture
foreign activity
DNase, RNase, protease, none detected
storage temp.
room temp
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