Brilliant Green Bile Lactose Broth 500g

Description

Synonyms: BRILA-Broth, Brilliant Green Bile Bouillon 2%

This culture medium complies with the recommendations of the International Dairy Federation (Internationaler Milchwirtschaftsverband) (FIL-IDF) (1985), the Standard Methods for Examination of Water and Wastewater (1998), the International Organization for Standardization (ISO) (1979) and the DIN Norm 10172.

Mode of Action
Bile and brilliant green almost completely inhibit the growth of the undesired microbial flora including lactose-degrading clostridia (e.g. Cl. perfringens) (MACKENZIE et al. 1948). The fermentation of lactose with gas formation indicates the presence of E. coli and other faecal coliform organisms and is established by using DURHAM tubes. Other non-faecal coliform bacterial also grow in this medium but mostly do not produce any gas.

Typical Composition (g/litre)
Peptone 10.0;
lactose 10.0;
ox bile, dried 20.0;
brilliant green 0.0133.

Preparation
Suspend 40 g/litre, fill into test tubes fitted with DURHAM tubes, autoclave (15 min at 121°C).
pH: 7.2 ± 0.2 at 25 °C.
The prepared broth is clear and green

Experimental Procedure and Evaluation
Inoculate the tubes.
Incubation: 24-48 hours at 35 °C or at the specified temperature (aerobically).
The E. coli titre refers to the smallest volume of sample material in which gas formation can be detected. Differentiation of the culture should also be performed to confirm the results obtained.