DNase test agar with toluidine blue 500g

Description

Application
Recommended for the detection of deoxyribonuclease activity of bacteria and fungi with the indicator toluidine blue.

Components
Ingredients (g/L)
Tryptose, 20.00
Deoxyribonucleic acid (DNA), 2.00
Sodium chloride, 5.00
Toluidine blue, 0.10
Agar, 15.00

Preparation Note
Suspend 42.1 g of DNase Test Agar with Toluidine Blue in 1000 ml of distilled water. Heat with frequent agitation to dissolve the medium completely. Sterilize by autoclaving at 12 to 15 lbs. pressure (118°C to 121°C) for 15 minutes. Cool to 45°C and pour into sterile petri plates.