Modified tryptone soy broth, 500g

Description

Application
Modified tryptone soy broth is recommended as an enrichment medium for the detection of Escherichia coli O157:H7 from food and animal feeding stuffs.

Preparation Note
Suspend 33 grams of Modified tryptone soy broth in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 minutes. Cool to 45-50 °C and aseptically add rehydrated contents of 1 vial of VCC Selective Supplement (Cat. No. No. 80704). Mix well and dispense as desired.

PROPERTIES

sterility
non-sterile

form
powder

shelf life
limited shelf life, expiry date on the label

composition
bile salts mixture, 1.5 g/L
casein enzymic hydrolysate, 17 g/L
dipotassium hydrogen phosphate, 4 g/L
D-glucose, 2.5 g/L
papaic digest of soyabean meal, 3 g/L
sodium chloride, 5 g/L

manufacturer/tradename
NutriSelect® Plus

technique(s)
microbiological culture: suitable

final pH
7.3±0.2 (25 °C)

application(s)
food and beverages
pathogen testing
vitamins, nutraceuticals, and natural products
microbiology

suitability
selective for enterovirulent E. coli (EEC)