Description
Mode of Action
The culture medium is optimally adapted to the special requirements of the microbial flora present in citrus juices (e.g. Bacillus, Lactobacillus, Leuconostoc species, fungi, etc.) due to the fact that it contains orange extract. It is therefore especially useful for the production control in the fruit juice industry
(HAYS and TIESTER 1952).
Typical Composition (g/litre)
Peptone from casein 10.0;
yeast extract 3.0;
orange extract 5.0;
D(+)glucose 4.0;
di-potassium hydrogen phosphate 3.0;
agar agar 17.0.
Preparation
Suspend 42 g/litre, autoclave under mild conditions (15 min at 115 °C). Do not overheat. Pour plates.
pH: 5.5 ± 0.2 at 25 °C.
The plates are clear to opalescent and yellowish-brown.
Experimental Procedure and Evaluation
Incubate the inoculated culture medium for up to 4 days at 28 °C aerobically; if fungi are suspected to be present, incubate for up to 5 days. Determine the microbial count. Further tests can be performed to differentiate and identify the colonies.
PROPERTIES
form
medium granules (dehydrated (DCM))
manufacturer/tradename
GranuCult®
technique(s)
microbiological culture: suitable
pH
5.4-5.6 (37 °C, 42 g/L in H2O, after autoclaving)
solubility
42 g/L
bulk density
700 kg/m3
application(s)
food and beverages
microbiology
storage temp.
15-25°C
suitability
molds
yeasts
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