ReadyPlate™55 KIT Chromocult® CN Agar

Description

This medium complies with the specifications given by EN ISO 16266 for the detection and enumeration of Pseudomonas aeruginosa in water using the membrane filtration technique.

Mode of Action
The peptone mixture in the media allows a broad spectrum of Pseudomonas to grow. The amount of potassium sulfate and magnesium chloride supports enhances the pigment production of Pseudomonas aeruginosa.

Typical Composition

Enzymatic digest of gelatine:    16 g/l

Enzymatic digest of casein:     10 g/l

Potassium sulfate:    10 g/l

Magnesium chloride:    1.4 g/l

Agar-agar* : 11 g/l

Water:   n/a

Glycerol:    10 ml

Cetrimide 0.2 g/l

Nalidixic acid:    0.015 g/l

pH at 25 °C:    7.1 ± 0.2

* Agar-agar is equivalent to other different terms of agar.

Experimental Procedure and Evaluation
Depend on the purpose for which the medium is used. Each plate is provided with a label including a data matrix code for paperless plate identification. The code consists of a two-dimensional 20-digit serial number, which harbors the following information: digits 1-3: here code 228 (corresponds to article 146709); digits 4-9: lot number; digits
10-14: batch specific individual number; digits 15-20: expiration date (YY/MM/DD).

Please check each agar plate before using it on sterility and pay attention to aseptic handling in order to avoid false positive results.
Following the procedure for direct enumeration given by EN ISO 16266, inoculate Pseudomonas CN agar plates using the membrane filtration technique.
Incubate at 34-38 °C for 40-48 hours aerobically.
Examine the membrane filters for growth after 20-24h and 40-48 h.
Count all colonies that produce a blue/green (pyocyanin) colour as confirmed
Pseudomonas aeruginosa.
For counting and confirmation of fluorescent colonies (under UV light) and/or reddish brown pigmented colonies follow the procedure given by EN ISO 16266.