Description
General description
YPD Broth is a nutritious general liquid growth medium commonly used for the maintenance and propagation of Pichia pastoris, Saccharomyces cerevisiae, and other yeasts. It is a medium used for molecular cloning, protein expression, or as enrichment media. Peptic digest of animal tissue provides nitrogenous nutrients. Yeast extract provides nitrogenous nutrients as well as Vitamin B Complex. Dextrose provides the carbohydrate and energy source to support the growth of S. cerevisiae.
Application
YPD Broth is recommended for the growth of Saccharomyces cerevisiae for molecular biology purposes. This medium supports the growth of most heterotrophic microorganisms but due to their simple composition, they have been adopted as the basal media for the routine cultivation of yeasts. General methods in yeast genetics specify using Yeast Extract Peptone- Dextrose (YPD) medium for cultivating S. cerevisiae and other yeasts.
Packaging
250 g in poly bottle
1 kg in poly bottle
Quantity
50 g per liter
Preparation Note
1. Suspend 50 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.
2. Autoclave for 15 minutes at 121°C.
PROPERTIES
sterility
non-sterile
form
powder
composition
Bacteriological peptone, 20 g/L
Glucose, 20 g/L
Yeast extract, 10 g/L
packaging
poly bottle of 1 kg
poly bottle of 250 g
manufacturer/tradename
NutriSelect® Basic
technique(s)
microbiological culture: suitable
color
beige
pH
6.3-6.7
application(s)
environmental
food and beverages
microbiology
suitability
nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.
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